2.43
1.65
1.98
0.73
1.88
1.81
2.43
2.2 Substaintí caighdeánacha a úsáidtear i gcuar calabrúcháin dáileadh mais mhóilíneach choibhneasta: inslin, micopeiptídí, glicín-glicín-tíoróisín-airginín, glicín-glicín-glicín
3 Ionstraim agus trealamh
23.2
21.4
22.2
16.1
22.3
20.8
23.9
27.5
Tríd is tríd, tá cion na n-aimínaigéad i dtáirgí Sustar níos airde ná an cion i dtáirgí Zinpro.
Cuid 8 Éifeachtaí úsáide
Éifeachtaí foinsí éagsúla mianraí rian ar fheidhmíocht táirgthe agus ar cháilíocht uibheacha cearca breithe sa tréimhse breithe déanach
Próiseas Táirgthe
Teicneolaíocht chealaithe spriocdhírithe
Teicneolaíocht eiblithe lomadh
Teicneolaíocht spraeála brú agus triomú
Teicneolaíocht cuisniúcháin agus dí-thaisiúcháin
Teicneolaíocht rialaithe comhshaoil ardleibhéil
Aguisín A: Modhanna chun dáileadh mais mhóilíneach choibhneasta peiptídí a chinneadh
Glacadh leis an gcaighdeán: GB/T 22492-2008
1 Prionsabal Tástála:
Cinneadh é trí chrómatagrafaíocht scagacháin glóthaí ardfheidhmíochta. Is é sin le rá, ag baint úsáide as líontóir scagach mar chéim sheasta, bunaithe ar an difríocht i méid mais mhóilíneach choibhneasta na gcomhpháirteanna sampla le haghaidh deighilte, a braitheadh ag an nasc peiptíde den tonnfhad ionsúcháin ultraivialait de 220nm, ag baint úsáide as na bogearraí próiseála sonraí tiomnaithe chun dáileadh mais mhóilíneach choibhneasta a chinneadh trí chrómatagrafaíocht scagacháin glóthaí (i.e., na bogearraí GPC), próiseáladh na crómatagram agus a gcuid sonraí, ríomhadh iad chun méid mais mhóilíneach choibhneasta an pheiptíde pónaire soighe agus an raon dáilte a fháil.
2. Imoibritheoirí
Ba cheart don uisce turgnamhach sonraíocht an uisce thánaisteach i GB/T6682 a chomhlíonadh, agus úsáid imoibrithe, seachas forálacha speisialta, a bheith íon ó thaobh anailíse de.
2.1 I measc na n-imoibrithe tá aicéatóinítríl (íon ó thaobh crómatagrafaíochta de), aigéad trífhluairaicéiteach (íon ó thaobh crómatagrafaíochta de),
2.2 Substaintí caighdeánacha a úsáidtear i gcuar calabrúcháin dáileadh mais mhóilíneach choibhneasta: inslin, micopeiptídí, glicín-glicín-tíoróisín-airginín, glicín-glicín-glicín
3 Ionstraim agus trealamh
3.1 Crómatagrafaíocht Leachtach Ardfheidhmíochta (HPLC): stáisiún oibre crómatagrafaíochta nó comhtháthaitheoir le brathadóir UV agus bogearraí próiseála sonraí GPC.
3.2 Aonad scagacháin agus díghásála folúis céime soghluaiste.
3.3 Cothromaíocht leictreonach: luach céimnithe 0.000 1g.
4 chéim oibriúcháin
4.1 Coinníollacha crómatagrafaíochta agus turgnaimh oiriúnaithe córais (coinníollacha tagartha)
- 4.1.1 Colún crómatagrafaíochta: TSKgelG2000swxl300 mm × 7.8 mm (trastomhas istigh) nó colúin glóthacha eile den chineál céanna le feidhmíocht chomhchosúil atá oiriúnach chun próitéiní agus peiptídí a chinneadh.
- 4.1.2 Céim shoghluaiste: Aicéatóinítríl + uisce + aigéad trífhluairaicéiteach = 20 + 80 + 0.1.
- 4.1.3 Tonnfhad braite: 220 nm.
- 4.1.4 Ráta sreafa: 0.5 mL/nóim.
- 4.1.5 Am braite: 30 nóiméad.
- 4.1.6 Toirt insteallta samplach: 20μL.
- 4.1.7 Teocht an cholúin: teocht an tseomra.
- 4.1.8 Chun go gcomhlíonfadh an córas crómatagrafaíochta na ceanglais bhrathadóireachta, foráladh faoi na coinníollacha crómatagrafaíochta thuas, nach mbeadh éifeachtúlacht an cholúin crómatagrafaíochta glóthaí, i.e., líon teoiriciúil na plátaí (N), níos lú ná 10000 arna ríomh ar bhonn bhuaicphointí an chaighdeáin trípheiptíde (Glicín-Glicín-Glicín).
- 4.2 Táirgeadh cuar caighdeánach mais mhóilíneach choibhneasta
- Ullmhaíodh na tuaslagáin chaighdeánacha peiptíde mais mhóilíneach choibhneasta éagsúla thuas le tiúchan maise de 1 mg / mL trí mheaitseáil céime soghluaiste, meascadh iad i gcion áirithe, agus ansin scagadh iad trí scannán céime orgánaí le méid póire 0.2 μm ~ 0.5 μm agus instealladh isteach sa sampla iad, agus ansin fuarthas crómatagram na gcaighdeán. Fuarthas cuar calabrúcháin mais mhóilíneach choibhneasta agus a gcothromóidí trí logartam na maise móilíneacha coibhneasta a phlota i gcoinne am coinneála nó trí aischéimniú líneach.
4.3 Cóireáil shamplach
Meáigh 10mg den sampla go cruinn i bhfleascán toirtmhéadrach 10mL, cuir beagán céime soghluaiste leis, croith go ultrasonaic ar feadh 10 nóiméad, ionas go mbeidh an sampla tuaslagtha go hiomlán agus measctha, caolaithe leis an gcéim shoghluaiste go dtí an scála, agus ansin scagtha trí scannán céime orgánaí le méid póire de 0.2μm ~ 0.5μm, agus rinneadh anailís ar an scagáit de réir na gcoinníollacha crómatagrafaíochta in A.4.1.
- 5. Ríomh dáileadh mais mhóilíneach choibhneasta
- Tar éis anailís a dhéanamh ar an tuaslagán samplach a ullmhaíodh i 4.3 faoi na coinníollacha crómatagrafaíochta de 4.1, is féidir mais mhóilíneach choibhneasta an tsampla agus a raon dáilte a fháil trí shonraí crómatagrafaíochta an tsampla a chur in ionad an chuar calabrúcháin 4.2 le bogearraí próiseála sonraí GPC. Is féidir dáileadh na maiseanna móilíneacha coibhneasta de na peiptídí éagsúla a ríomh tríd an modh normalúcháin achar buaic, de réir na foirmle: X=A/A iomlán×100
- Sa fhoirmle: X - An codán maise de pheiptíd mais mhóilíneach choibhneasta sa pheiptíd iomlán sa sampla, %;
- A - Achar buaicphointe peiptíde mais mhóilíneach choibhneasta;
- Iomlán A - suim na mbuaic-achar de gach peiptíd mais mhóilíneach choibhneasta, ríofa go dtí an áit dheachúil amháin.
- 6 In-athdhéantacht
- Ní rachaidh an difríocht absalóideach idir dhá chinneadh neamhspleácha a fhaightear faoi choinníollacha in-athdhéantachta thar 15% de mheán uimhríochtúil an dá chinneadh.
- Aguisín B: Modhanna chun Aimínaigéid Saora a Chinneadh
- Glacadh leis an gcaighdeán: Q/320205 KAVN05-2016
- 1.2 Imoibrithe agus ábhair
- Aigéad aicéiteach oighreach: íon go hanailíseach
- Aigéad perclórach: 0.0500 mol/L
- Táscaire: táscaire criostail violet 0.1% (aigéad aicéiteach oighreach)
- 2. Cinneadh aimínaigéid saora
Triomaíodh na samplaí ag 80°C ar feadh 1 uair an chloig.
Cuir an sampla i gcoimeádán tirim le fuarú go nádúrtha go teocht an tseomra nó le fuarú síos go teocht inúsáidte.Meáigh thart ar 0.1 g den sampla (cruinn go 0.001 g) isteach i bhfleascán cónúil tirim 250 mL.Téigh ar aghaidh go tapa go dtí an chéad chéim eile chun a chinntiú nach n-ionsóidh an sampla taise comhthimpeallach.Cuir 25 mL d'aigéad aicéiteach oighreach leis agus measc go maith ar feadh 5 nóiméad ar a mhéad.Cuir 2 bhraon den táscaire criostail violet leisTitrátaigh le tuaslagán títrithe caighdeánach 0.0500 mol / L (±0.001) d'aigéad perclórach go dtí go n-athraíonn an tuaslagán ó chorcra go dtí an pointe deiridh.
Taifead an méid tuaslagáin chaighdeánaigh a ídíodh.
- Déan an tástáil bán ag an am céanna.
- 3. Ríomh agus torthaí
- Déantar cion an aimínaigéid shaor X san imoibrí a chur in iúl mar chodán maise (%) agus ríomhtar é de réir na foirmle: X = C × (V1-V0) × 0.1445/M × 100%, sa fhoirmle seo:
- C - Tiúchan tuaslagáin aigéid pheirclóraigh chaighdeánaigh i móil in aghaidh an lítir (mól/L)
- V1 - An toirt a úsáidtear le haghaidh tíotrádú samplaí le tuaslagán caighdeánach aigéad perclórach, i millilítear (mL).
- Vo - An toirt a úsáidtear le haghaidh bán-titrithe le tuaslagán caighdeánach aigéad perclórach, i millilítear (mL);
M - Mais an tsampla, i ngraim (g).
| 0.1445: Meánmhais aimínaigéid atá coibhéiseach le 1.00 mL de thuaslagán caighdeánach aigéid pheirclóraigh [c (HClO4) = 1.000 mol / L]. | 4.2.3 Tuaslagán tíotráite caighdeánach sulfáit cheiriam: tiúchan c [Ce (SO4) 2] = 0.1 mol/L, ullmhaithe de réir GB/T601. | |
| Glacadh le caighdeáin: Q/70920556 71-2024 | 1. Prionsabal cinntiúcháin (Fe mar shampla) | Tá intuaslagthacht an-íseal ag coimpléisc iarainn aimínaigéid in eatánól anhidriúil agus tá iain miotail shaora intuaslagtha in eatánól anhidriúil, baineadh úsáid as an difríocht intuaslagthachta idir an dá cheann in eatánól anhidriúil chun ráta cealaithe coimpléisc iarainn aimínaigéid a chinneadh. |
| Sa fhoirmle: V1 - toirt an tuaslagáin chaighdeánaigh sulfáit cheiriam a ídítear le haghaidh tíotrádú an tuaslagáin tástála, mL; | Eatánól ainhidriúil; tá an chuid eile mar a chéile le clásal 4.5.2 i GB/T 27983-2011. | 3. Céimeanna anailíse |
| Déan dhá thriail ag an am céanna. Meáigh 0.1g den sampla triomaithe ag 103±2℃ ar feadh 1 uair an chloig, cruinn go 0.0001g, cuir 100mL d'eatánól anhidriúil leis chun é a thuaslagadh, scag, scag an t-iarmhar agus nite le 100mL d'eatánól anhidriúil ar feadh trí huaire ar a laghad, ansin aistrigh an t-iarmhar isteach i bhfleascán cónúil 250mL, cuir 10mL de thuaslagán aigéid sulfairigh leis de réir clásal 4.5.3 i GB/T27983-2011, agus ansin déan na céimeanna seo a leanas de réir clásal 4.5.3 “Teas chun é a thuaslagadh agus ansin lig dó fuarú” i GB/T27983-2011. Déan an tástáil bán ag an am céanna. | 4. Cinneadh an ábhair iarainn iomláin | 4.1 Is ionann prionsabal an chinnidh agus clásal 4.4.1 i GB/T 21996-2008. |
4.2. Imoibrithe & Tuaslagáin
| 4.2.1 Aigéad measctha: Cuir 150mL d'aigéad sulfarach agus 150mL d'aigéad fosfarach le 700mL uisce agus measc go maith. | 4.2.2 Tuaslagán táscaire sulfónáite défheinilaimíne sóidiam: 5g/L, ullmhaithe de réir GB/T603. | 4.2.3 Tuaslagán tíotráite caighdeánach sulfáit cheiriam: tiúchan c [Ce (SO4) 2] = 0.1 mol/L, ullmhaithe de réir GB/T601. | |
| 4.3 Céimeanna anailíse | Déan dhá thriail ag an am céanna. Meáigh 0.1g den sampla, cruinn go 020001g, cuir i bhfleascán cónúil 250mL é, cuir 10mL d'aigéad measctha leis, tar éis a thuaslagadh, cuir 30ml uisce agus 4 bhraon de thuaslagán táscaire dé-ainilín sulfónáite sóidiam leis, agus ansin déan na céimeanna seo a leanas de réir clásal 4.4.2 i GB/T21996-2008. Déan an tástáil bán ag an am céanna. | 4.4 Léiriú na dtorthaí | Rinneadh an cion iarainn iomlán X1 de na coimpléisc iarainn aimínaigéid i dtéarmaí codán maise an iarainn, an luach a léirítear i %, a ríomh de réir fhoirmle (1): |
| X1=(V-V0)×C×M×10-3×100 | V0 - tuaslagán caighdeánach sulfáit cheiriam a ídíodh le haghaidh tíotrádú tuaslagáin bhán, mL; | V0 - tuaslagán caighdeánach sulfáit cheiriam a ídíodh le haghaidh tíotrádú tuaslagáin bhán, mL; | C - Tiúchan iarbhír tuaslagáin chaighdeánaigh sulfáit cheiriam, mol/L5. Ríomh an chion iarainn i gcealáitíRinneadh an cion iarainn X2 sa chealáit i dtéarmaí chodán maise an iarainn, an luach a léirítear i %, a ríomh de réir na foirmle: x2 = ((V1-V2) × C × 0.05585)/m1 × 100 |
| Sa fhoirmle: V1 - toirt an tuaslagáin chaighdeánaigh sulfáit cheiriam a ídítear le haghaidh tíotrádú an tuaslagáin tástála, mL; | V2 - tuaslagán caighdeánach sulfáit cheiriam a ídíodh le haghaidh tíotrádú tuaslagáin bhán, mL;nom1-Mais an tsampla, g. Glac meán uimhríochtúil thorthaí an chinnidh chomhthreomhair mar thorthaí an chinnidh, agus ní mó ná 0.3% an difríocht absalóideach idir thorthaí an chinnidh chomhthreomhair. | 0.05585 - mais iarainn fheiriúil arna sloinneadh i ngraim atá coibhéiseach le 1.00 mL de thuaslagán caighdeánach sulfáit cheiriam C[Ce(SO4)2.4H20] = 1.000 mol/L.nom1-Mais an tsampla, g. Glac meán uimhríochtúil thorthaí an chinnidh chomhthreomhair mar thorthaí an chinnidh, agus ní mó ná 0.3% an difríocht absalóideach idir thorthaí an chinnidh chomhthreomhair. | 6. Ríomh an ráta cealaitheRáta cealaithe X3, an luach léirithe i %, X3 = X2/X1 × 100Aguisín C: Modhanna chun ráta cealaithe Zinpro a Chinneadh |
Glacadh leis an gcaighdeán: Q/320205 KAVNO7-2016
1. Imoibrithe agus ábhair
a) Aigéad aicéiteach oighreach: íon go hanailíseach; b) Aigéad peirclórach: 0.0500mol/L; c) Táscaire: táscaire criostail violet 0.1% (aigéad aicéiteach oighreach)
2. Cinneadh aimínaigéid saora
2.1 Triomaíodh na samplaí ag 80°C ar feadh 1 uair an chloig.
2.2 Cuir an sampla i gcoimeádán tirim le fuarú go nádúrtha go teocht an tseomra nó le fuarú síos go teocht inúsáidte.
2.3 Meáigh thart ar 0.1 g den sampla (cruinn go 0.001 g) isteach i bhfleascán cónúil tirim 250 mL
2.4 Téigh ar aghaidh go tapa go dtí an chéad chéim eile chun a chinntiú nach n-ionsóidh an sampla taise comhthimpeallach.
2.5 Cuir 25mL d'aigéad aicéiteach oighreach leis agus measc go maith ar feadh tréimhse nach faide ná 5 nóiméad.
2.6 Cuir 2 bhraon den táscaire criostail violet leis.
2.7 Déan tíotrátáil le tuaslagán tíotráthaithe caighdeánach 0.0500mol/L (±0.001) d'aigéad perclórach go dtí go n-athraíonn an tuaslagán ó chorcra go glas ar feadh 15 soicind gan dath a athrú mar chríochphointe.
2.8 Taifead an méid tuaslagáin chaighdeánaigh a ídíodh.
2.9 Déan an tástáil bán ag an am céanna.
- 3. Ríomh agus torthaí
- Catalóinis
- Physicochemical parameters
V1 - An toirt a úsáidtear le haghaidh tíotrádú samplaí le tuaslagán caighdeánach aigéad perclórach, i millilítear (mL).
Vo - An toirt a úsáidtear le haghaidh bán-titrithe le tuaslagán caighdeánach aigéad perclórach, i millilítear (mL);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Seoladh: Uimh.147 Bóthar Qingpu, Baile Shouan, Contae Pujiang, Cathair Chengdu, Cúige Sichuan, an tSín
Fón: 86-18880477902
Táirgí
mianraí rian neamhorgánacha
- Mianraí rian orgánacha
- Svahaílis
- Seirbhís saincheaptha
- Naisc thapa
Próifíl na Cuideachta
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Gúisearáitis | Cliceáil le haghaidh fiosrúcháin | © Cóipcheart - 2010-2025: Gach ceart ar cosaint. | Léarscáil an tSuímh CUARDACH BARR Fón |
| Teil | 86-18880477902 | Iávais | Ríomhphost |
| 8618880477902 | Sínis | Fraincis | |
| Bird | Sínis | Fraincis | Gearmáinis Spáinnis |
| Aquatic animals | Seapáinis | Cóiréach | Araibis Gréigis |
| Tuircis | Iodáilis | ||
| Ruminant animal g/head day | January 0.75 | Indinéisis Afracáinis Sualainnis |
Polainnis
- Bascais
- Catalóinis
- Physicochemical parameters
Hiondúis
Laosach
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Seona
Bulgáiris
- Cebuano
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- Cróitis
Ollainnis
| Application object | Urdu Vítneamach | Content in full-value feed (mg/kg) | Efficacy |
| Gúisearáitis | Háítíoch | Hausa | Cinéarvandais Hmong Ungáiris |
| Piglets and fattening pigs | Igbo | Iávais | Cannadais Ciméireach Cúrdaí |
| Cirgeasais | Laidin | ||
| Bird | 300～400 | 45～60 | Macadóinis Malaeis Mailéalaimis |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Ioruais
- Paistis
- Appearance: brownish-yellow granules
- Physicochemical parameters
Seirbis
Sesoto
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Seona
Sindhi
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
Svahaílis
Táidsícis
Tamailis
Teileagúis
Téalainnis
| Application object | Urdu Vítneamach | Content in full-value feed (mg/kg) | Efficacy |
| Giúdais | Iarúba | Súlúis | Cinéarvandais Oriya Tuircméinis |
| Úigiúrach | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1.52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
